IHC and ISH
Immunohistochemistry (IHC) and in situ hybridization (ISH) services are provided to customers with specific needs for tissue array experiments. These analyses are performed to localize the protein/gene of interest by using customer-supplied antibodies, DNA fragments or oligonucleotides on normal and diseased human tissues selected from US Biomax's vast selection of tissue arrays and tissue bank. These services are highly cost-effective and conducted to the highest standards to assist you in understanding function of your genes/proteins or in identification and validation of your potential diagnostic and therapeutic targets. The results are presented as a publishable, detailed pathology report accompanied by high-resolution digital photographs.
US Biomax uses an automated IHC stainer (Ventana) for high staining quality that ensures reproducible results. The research platform allows for customization of the reagents and protocol for best results. We offer multiple levels of detection amplification, allowing for highly sensitive assays to ensure that there are no false negatives. The use of a Streptavidin-Horseradish Peroxidase (HRP) minimizes nonspecific binding compared to biotin-based detection systems. For more details about high-sensitivity detection, please refer to [1], and view example staining for ALK detection provided here.
Sample results:
Target | TMA | View hi-res |
---|---|---|
CD3 | LY2084 | View hi-res |
CD20 | LY2084 | View hi-res |
ER | BR2082b | View hi-res |
PR | BR2082b | View hi-res |
HER2 | BR2082b | View hi-res |
ALK | Lung (NSCLC) | View hi-res |
PD-L1 | Lung (NSCLC) | View hi-res |
LKB1 | Lung Cell Lines (NSCLC) | View hi-res |
Image Analysis
US Biomax offers the use of Visiopharm for powerful and customizable image analysis. The following services are available for routine analysis:
- Automated quantification of cytoplasm, membrane, or nucleus staining
- Virtual double/triple staining by aligning features on individual cores. Applications include co-localization studies, and automated quantification of tumor regions only.
- A wide variety of algorithms for specific biomarkers shared by others at the Visiopharm App Center
References:
- New Methods for ALK Status Diagnosis in Non–Small-Cell Lung Cancer: An Improved ALK Immunohistochemical Assay and a new, Brightfield, dual ALK IHC-in situ hybridization assay.
Nitta, Hiroaki et al., Journal of Thoracic Oncology , Volume 8 , Issue 8 , 1019 - 1031