ATF 1 10 5 Type=GenePix ArrayList V1.0 BlockCount=6 BlockType=0 URL=http://www.fullmoonbiosystems.com "Block1=2247, 11217, 250, 32, 600, 6, 600" "Block2=2247, 15717, 250, 32, 600, 6, 600" "Block3=2247, 20217, 250, 32, 600, 6, 600" "Block4=2247, 24717, 250, 32, 600, 6, 600" "Block5=2247, 29217, 250, 32, 600, 6, 600" "Block6=2247, 33717, 250, 32, 600, 6, 600" Block Row Column Name ID 1 1 1 Positive Marker P 1 1 2 Empty E 1 1 3 Empty E 1 1 4 Elk-1 (Phospho-Ser383) 1 1 1 5 Raf1 (Phospho-Ser259) 2 1 1 6 MEK-2 (Phospho-Thr394) 3 1 1 7 Elk1 (Phospho-Ser389) 4 1 1 8 Elk1 (Phospho-Thr417) 5 1 1 9 AKT (Phospho-Ser473) 6 1 1 10 AKT (Phospho-Thr308) 7 1 1 11 FAK (Phospho-Tyr861) 8 1 1 12 BAD (Phospho-Ser112) 9 1 1 13 BAD (Phospho-Ser136) 10 1 1 14 BAD (Phospho-Ser155) 11 1 1 15 VEGFR2 (Phospho-Tyr1175) 12 1 1 16 VEGFR2 (Phospho-Tyr1214) 13 1 1 17 VEGFR2 (Phospho-Tyr951) 14 1 1 18 Paxillin (Phospho-Tyr118) 15 1 1 19 Src (Phospho-Tyr418) 16 1 1 20 FKHR (Phospho-Ser256) 17 1 1 21 FAK (Phospho-Tyr925) 18 1 1 22 AKT2 (Phospho-Ser474) 19 1 1 23 FKHR (Phospho-Ser319) 20 1 1 24 MKK3 (Phospho-Ser189 21 1 1 25 Src (Phospho-Tyr529) 22 1 1 26 eNOS (Phospho-Ser1177) 23 1 1 27 MEK1 (Phospho-Ser221) 24 1 1 28 HSP27 (Phospho-Ser15) 25 1 1 29 Paxillin (Phospho-Tyr31) 26 1 1 30 Raf1 (Phospho-Ser338) 27 1 1 31 MEK1 (Phospho-Ser217) 28 1 1 32 eNOS (Phospho-Thr495) 29 2 1 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 1 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 1 3 HSP27 (Phospho-Ser78) 32 2 1 4 HSP27 (Phospho-Ser82) 33 2 1 5 P38 MAPK (Phospho-Thr180) 34 2 1 6 P38 MAPK (Phospho-Tyr182) 35 2 1 7 MEK1 (Phospho-Thr291) 36 2 1 8 PKC delta (Phospho-Ser645) 37 2 1 9 PKC theta (Phospho-Ser676) 38 2 1 10 HSP90B (Phospho-Ser254) 39 2 1 11 PKC zeta (Phospho-Thr410) 40 2 1 12 Shc (Phospho-Tyr349) 41 2 1 13 Shc (Phospho-Tyr427) 42 2 1 14 Elk-1 (Ab-383) 43 2 1 15 Raf1 (Ab-259) 44 2 1 16 MEK-2 (Ab-394) 45 2 1 17 Elk1 (Ab-389) 46 2 1 18 Elk1 (Ab-417) 47 2 1 19 AKT (Ab-473) 48 2 1 20 AKT (Ab-308) 49 2 1 21 BAD (Ab-112) 50 2 1 22 BAD (Ab-136) 51 2 1 23 BAD (Ab-155) 52 2 1 24 FAK (Ab-861) 53 2 1 25 VEGFR2 (Ab-1175) 54 2 1 26 VEGFR2 (Ab-1214) 55 2 1 27 VEGFR2 (Ab-951) 56 2 1 28 Paxillin (Ab-118) 57 2 1 29 Src (Ab-418) 58 2 1 30 MKK3 (Ab-189) 59 2 1 31 FKHR (Ab-256) 60 2 1 32 HSP27 (Ab-15) 61 3 1 1 FAK (Ab-925) 62 3 1 2 AKT2 (Ab-474) 63 3 1 3 FKHR (Ab-319) 64 3 1 4 Src (Ab-529) 65 3 1 5 eNOS (Ab-1177) 66 3 1 6 MEK1 (Ab-221) 67 3 1 7 Paxillin (Ab-31) 68 3 1 8 Raf1 (Ab-338) 69 3 1 9 MEK1 (Ab-217) 70 3 1 10 FAK (Ab-397) 71 3 1 11 eNOS (Ab-495) 72 3 1 12 p44/42 MAP Kinase (Ab-202) 73 3 1 13 p44/42 MAP Kinase (Ab-204) 74 3 1 14 HSP27 (Ab-78) 75 3 1 15 HSP27 (Ab-82) 76 3 1 16 P38 MAPK (Ab-182) 77 3 1 17 MEK1 (Ab-291) 78 3 1 18 PKC delta (Ab-645) 79 3 1 19 PKC theta (Ab-676) 80 3 1 20 HSP90B (Ab-254) 81 3 1 21 PKC zeta (Ab-410) 82 3 1 22 Shc (Ab-349) 83 3 1 23 MKK6 (Phospho-Ser207) 84 3 1 24 FAK (Phospho-Tyr397) 85 3 1 25 MAPKAPK2 (Phospho-Thr334) 86 3 1 26 c-PLA2 (Phospho-Ser505) 87 3 1 27 AKT1 (Phospho-Thr450) 88 3 1 28 FAK (Phospho-Tyr576) 89 3 1 29 FAK (Phospho-Tyr407) 90 3 1 30 Raf1 (Phospho-Tyr341) 91 3 1 31 Raf1(Phospho-Ser621) 92 3 1 32 VEGFR-1 (Phospho-Tyr1333) 93 4 1 1 VEGFR2 (Phospho-Tyr1059) 94 4 1 2 AKT1 (Phospho-Thr72) 95 4 1 3 MAPKAPK-2 (Phospho-Thr222) 96 4 1 4 MEK1 (Phospho-Ser298) 97 4 1 5 MEK1 (Phospho-Thr286) 98 4 1 6 PKC alpha/beta II (Phospho-Thr638) 99 4 1 7 PKC delta (Phospho-Thr505) 100 4 1 8 PKC theta (Phospho-Thr538) 101 4 1 9 Ras-GRF1 (Phospho-Ser916) 102 4 1 10 p38 MAPK (Phospho-Tyr322) 103 4 1 11 PKC alpha (Phospho-Tyr657) 104 4 1 12 PKC epsilon (Phospho-Ser729) 105 4 1 13 PKC zeta (Phospho-Thr560) 106 4 1 14 AKT (Phospho-Tyr326) 107 4 1 15 BAD (Phospho-Ser91/128) 108 4 1 16 BAD (Phospho-Ser134) 109 4 1 17 Caspase 9 (Phospho-Tyr153) 110 4 1 18 Caspase 9 (Phospho-Ser144) 111 4 1 19 Caspase 9 (Phospho-Ser196) 112 4 1 20 ERK3 (Phospho-Ser189) 113 4 1 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 1 22 FAK (Phospho-Ser910) 115 4 1 23 GTPase activating protein (Phopspho-Ser387) 116 4 1 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 1 25 RapGEF1 (Phospho-Tyr504) 118 4 1 26 eNOS (Phospho-Ser615) 119 4 1 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 1 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 1 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 1 30 PKC beta/PKCB (Phospho-Ser661) 123 4 1 31 Src (Phospho-Ser75) 124 4 1 32 Src (Phospho-Tyr216) 125 5 1 1 GRB2 (Phospho-Ser159) 126 5 1 2 MAPKAPK2 (Phospho-Ser272) 127 5 1 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 1 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 1 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 1 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 1 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 1 8 VEGF R2 (Phospho-Tyr1054) 133 5 1 9 MKK6 (Ab-207) 134 5 1 10 MAPKAPK2 (Ab-334) 135 5 1 11 Shc (Ab-427) 136 5 1 12 c-PLA2 (Ab-505) 137 5 1 13 AKT1 (Ab-450) 138 5 1 14 FAK (Ab-576) 139 5 1 15 FAK (Ab-407) 140 5 1 16 Raf1 (Ab-341) 141 5 1 17 Raf1 (Ab-621) 142 5 1 18 VEGFR-1 (Ab-1333) 143 5 1 19 VEGFR2 (Ab-1059) 144 5 1 20 AKT1 (Ab-72) 145 5 1 21 MEK1 (Ab-298) 146 5 1 22 MEK1 (Ab286) 147 5 1 23 PKC alpha/beta II (Ab-638) 148 5 1 24 PKC delta (Ab-505) 149 5 1 25 PKC theta (Ab-538) 150 5 1 26 Ras-GRF1 (Ab-916) 151 5 1 27 p38 MAPK (Ab-322) 152 5 1 28 PKC alpha (Ab-657) 153 5 1 29 PKC epsilon (Ab-729) 154 5 1 30 PKC zeta (Ab-560) 155 5 1 31 AKT (Ab-326) 156 5 1 32 BAD (Ab-91/128) 157 6 1 1 BAD (Ab-134) 158 6 1 2 Caspase 9 (Ab-153) 159 6 1 3 Caspase 9 (Ab-144) 160 6 1 4 Caspase 9 (Ab-196) 161 6 1 5 ERK3 (Ab-189) 162 6 1 6 FAK (Ab-910) 163 6 1 7 GTPase activating protein (Ab-387) 164 6 1 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 1 9 eNOS (Ab-615) 166 6 1 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 1 11 AKT1/2/3 (Ab-315) 168 6 1 12 MKK3/MAP2K3 (Ab-222) 169 6 1 13 PKC beta/PKCB (Ab-661) 170 6 1 14 Raf1 (Ab-289) 171 6 1 15 Src (Ab-75) 172 6 1 16 GRB2 (Ab-159) 173 6 1 17 MAPKAPK2 (Ab-272) 174 6 1 18 PLC-gamma 1 (Ab-771) 175 6 1 19 PLC-gamma 1 (Ab-783) 176 6 1 20 PLC-gamma 2 (Ab-753) 177 6 1 21 PLC-gamma 2 (Ab-1217) 178 6 1 22 VEGF R2 (Ab-1054) 179 6 1 23 ERK1/2 (N-term) 180 6 1 24 HSP90A (C-term) 181 6 1 25 RAS(p21 H and K) (inter) 182 6 1 26 IP3KA (C-term) 183 6 1 27 IP3KC (inter) 184 6 1 28 RASE (inter) 185 6 1 29 Beta actin A 6 1 30 GAPDH G 6 1 31 Negative Control N 6 1 32 Positive Marker P 1 2 1 Positive Marker P 1 2 2 Empty E 1 2 3 Empty E 1 2 4 Elk-1 (Phospho-Ser383) 1 1 2 5 Raf1 (Phospho-Ser259) 2 1 2 6 MEK-2 (Phospho-Thr394) 3 1 2 7 Elk1 (Phospho-Ser389) 4 1 2 8 Elk1 (Phospho-Thr417) 5 1 2 9 AKT (Phospho-Ser473) 6 1 2 10 AKT (Phospho-Thr308) 7 1 2 11 FAK (Phospho-Tyr861) 8 1 2 12 BAD (Phospho-Ser112) 9 1 2 13 BAD (Phospho-Ser136) 10 1 2 14 BAD (Phospho-Ser155) 11 1 2 15 VEGFR2 (Phospho-Tyr1175) 12 1 2 16 VEGFR2 (Phospho-Tyr1214) 13 1 2 17 VEGFR2 (Phospho-Tyr951) 14 1 2 18 Paxillin (Phospho-Tyr118) 15 1 2 19 Src (Phospho-Tyr418) 16 1 2 20 FKHR (Phospho-Ser256) 17 1 2 21 FAK (Phospho-Tyr925) 18 1 2 22 AKT2 (Phospho-Ser474) 19 1 2 23 FKHR (Phospho-Ser319) 20 1 2 24 MKK3 (Phospho-Ser189 21 1 2 25 Src (Phospho-Tyr529) 22 1 2 26 eNOS (Phospho-Ser1177) 23 1 2 27 MEK1 (Phospho-Ser221) 24 1 2 28 HSP27 (Phospho-Ser15) 25 1 2 29 Paxillin (Phospho-Tyr31) 26 1 2 30 Raf1 (Phospho-Ser338) 27 1 2 31 MEK1 (Phospho-Ser217) 28 1 2 32 eNOS (Phospho-Thr495) 29 2 2 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 2 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 2 3 HSP27 (Phospho-Ser78) 32 2 2 4 HSP27 (Phospho-Ser82) 33 2 2 5 P38 MAPK (Phospho-Thr180) 34 2 2 6 P38 MAPK (Phospho-Tyr182) 35 2 2 7 MEK1 (Phospho-Thr291) 36 2 2 8 PKC delta (Phospho-Ser645) 37 2 2 9 PKC theta (Phospho-Ser676) 38 2 2 10 HSP90B (Phospho-Ser254) 39 2 2 11 PKC zeta (Phospho-Thr410) 40 2 2 12 Shc (Phospho-Tyr349) 41 2 2 13 Shc (Phospho-Tyr427) 42 2 2 14 Elk-1 (Ab-383) 43 2 2 15 Raf1 (Ab-259) 44 2 2 16 MEK-2 (Ab-394) 45 2 2 17 Elk1 (Ab-389) 46 2 2 18 Elk1 (Ab-417) 47 2 2 19 AKT (Ab-473) 48 2 2 20 AKT (Ab-308) 49 2 2 21 BAD (Ab-112) 50 2 2 22 BAD (Ab-136) 51 2 2 23 BAD (Ab-155) 52 2 2 24 FAK (Ab-861) 53 2 2 25 VEGFR2 (Ab-1175) 54 2 2 26 VEGFR2 (Ab-1214) 55 2 2 27 VEGFR2 (Ab-951) 56 2 2 28 Paxillin (Ab-118) 57 2 2 29 Src (Ab-418) 58 2 2 30 MKK3 (Ab-189) 59 2 2 31 FKHR (Ab-256) 60 2 2 32 HSP27 (Ab-15) 61 3 2 1 FAK (Ab-925) 62 3 2 2 AKT2 (Ab-474) 63 3 2 3 FKHR (Ab-319) 64 3 2 4 Src (Ab-529) 65 3 2 5 eNOS (Ab-1177) 66 3 2 6 MEK1 (Ab-221) 67 3 2 7 Paxillin (Ab-31) 68 3 2 8 Raf1 (Ab-338) 69 3 2 9 MEK1 (Ab-217) 70 3 2 10 FAK (Ab-397) 71 3 2 11 eNOS (Ab-495) 72 3 2 12 p44/42 MAP Kinase (Ab-202) 73 3 2 13 p44/42 MAP Kinase (Ab-204) 74 3 2 14 HSP27 (Ab-78) 75 3 2 15 HSP27 (Ab-82) 76 3 2 16 P38 MAPK (Ab-182) 77 3 2 17 MEK1 (Ab-291) 78 3 2 18 PKC delta (Ab-645) 79 3 2 19 PKC theta (Ab-676) 80 3 2 20 HSP90B (Ab-254) 81 3 2 21 PKC zeta (Ab-410) 82 3 2 22 Shc (Ab-349) 83 3 2 23 MKK6 (Phospho-Ser207) 84 3 2 24 FAK (Phospho-Tyr397) 85 3 2 25 MAPKAPK2 (Phospho-Thr334) 86 3 2 26 c-PLA2 (Phospho-Ser505) 87 3 2 27 AKT1 (Phospho-Thr450) 88 3 2 28 FAK (Phospho-Tyr576) 89 3 2 29 FAK (Phospho-Tyr407) 90 3 2 30 Raf1 (Phospho-Tyr341) 91 3 2 31 Raf1(Phospho-Ser621) 92 3 2 32 VEGFR-1 (Phospho-Tyr1333) 93 4 2 1 VEGFR2 (Phospho-Tyr1059) 94 4 2 2 AKT1 (Phospho-Thr72) 95 4 2 3 MAPKAPK-2 (Phospho-Thr222) 96 4 2 4 MEK1 (Phospho-Ser298) 97 4 2 5 MEK1 (Phospho-Thr286) 98 4 2 6 PKC alpha/beta II (Phospho-Thr638) 99 4 2 7 PKC delta (Phospho-Thr505) 100 4 2 8 PKC theta (Phospho-Thr538) 101 4 2 9 Ras-GRF1 (Phospho-Ser916) 102 4 2 10 p38 MAPK (Phospho-Tyr322) 103 4 2 11 PKC alpha (Phospho-Tyr657) 104 4 2 12 PKC epsilon (Phospho-Ser729) 105 4 2 13 PKC zeta (Phospho-Thr560) 106 4 2 14 AKT (Phospho-Tyr326) 107 4 2 15 BAD (Phospho-Ser91/128) 108 4 2 16 BAD (Phospho-Ser134) 109 4 2 17 Caspase 9 (Phospho-Tyr153) 110 4 2 18 Caspase 9 (Phospho-Ser144) 111 4 2 19 Caspase 9 (Phospho-Ser196) 112 4 2 20 ERK3 (Phospho-Ser189) 113 4 2 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 2 22 FAK (Phospho-Ser910) 115 4 2 23 GTPase activating protein (Phopspho-Ser387) 116 4 2 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 2 25 RapGEF1 (Phospho-Tyr504) 118 4 2 26 eNOS (Phospho-Ser615) 119 4 2 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 2 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 2 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 2 30 PKC beta/PKCB (Phospho-Ser661) 123 4 2 31 Src (Phospho-Ser75) 124 4 2 32 Src (Phospho-Tyr216) 125 5 2 1 GRB2 (Phospho-Ser159) 126 5 2 2 MAPKAPK2 (Phospho-Ser272) 127 5 2 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 2 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 2 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 2 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 2 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 2 8 VEGF R2 (Phospho-Tyr1054) 133 5 2 9 MKK6 (Ab-207) 134 5 2 10 MAPKAPK2 (Ab-334) 135 5 2 11 Shc (Ab-427) 136 5 2 12 c-PLA2 (Ab-505) 137 5 2 13 AKT1 (Ab-450) 138 5 2 14 FAK (Ab-576) 139 5 2 15 FAK (Ab-407) 140 5 2 16 Raf1 (Ab-341) 141 5 2 17 Raf1 (Ab-621) 142 5 2 18 VEGFR-1 (Ab-1333) 143 5 2 19 VEGFR2 (Ab-1059) 144 5 2 20 AKT1 (Ab-72) 145 5 2 21 MEK1 (Ab-298) 146 5 2 22 MEK1 (Ab286) 147 5 2 23 PKC alpha/beta II (Ab-638) 148 5 2 24 PKC delta (Ab-505) 149 5 2 25 PKC theta (Ab-538) 150 5 2 26 Ras-GRF1 (Ab-916) 151 5 2 27 p38 MAPK (Ab-322) 152 5 2 28 PKC alpha (Ab-657) 153 5 2 29 PKC epsilon (Ab-729) 154 5 2 30 PKC zeta (Ab-560) 155 5 2 31 AKT (Ab-326) 156 5 2 32 BAD (Ab-91/128) 157 6 2 1 BAD (Ab-134) 158 6 2 2 Caspase 9 (Ab-153) 159 6 2 3 Caspase 9 (Ab-144) 160 6 2 4 Caspase 9 (Ab-196) 161 6 2 5 ERK3 (Ab-189) 162 6 2 6 FAK (Ab-910) 163 6 2 7 GTPase activating protein (Ab-387) 164 6 2 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 2 9 eNOS (Ab-615) 166 6 2 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 2 11 AKT1/2/3 (Ab-315) 168 6 2 12 MKK3/MAP2K3 (Ab-222) 169 6 2 13 PKC beta/PKCB (Ab-661) 170 6 2 14 Raf1 (Ab-289) 171 6 2 15 Src (Ab-75) 172 6 2 16 GRB2 (Ab-159) 173 6 2 17 MAPKAPK2 (Ab-272) 174 6 2 18 PLC-gamma 1 (Ab-771) 175 6 2 19 PLC-gamma 1 (Ab-783) 176 6 2 20 PLC-gamma 2 (Ab-753) 177 6 2 21 PLC-gamma 2 (Ab-1217) 178 6 2 22 VEGF R2 (Ab-1054) 179 6 2 23 ERK1/2 (N-term) 180 6 2 24 HSP90A (C-term) 181 6 2 25 RAS(p21 H and K) (inter) 182 6 2 26 IP3KA (C-term) 183 6 2 27 IP3KC (inter) 184 6 2 28 RASE (inter) 185 6 2 29 Beta actin A 6 2 30 GAPDH G 6 2 31 Negative Control N 6 2 32 Positive Marker P 1 3 1 Positive Marker P 1 3 2 Empty E 1 3 3 Empty E 1 3 4 Elk-1 (Phospho-Ser383) 1 1 3 5 Raf1 (Phospho-Ser259) 2 1 3 6 MEK-2 (Phospho-Thr394) 3 1 3 7 Elk1 (Phospho-Ser389) 4 1 3 8 Elk1 (Phospho-Thr417) 5 1 3 9 AKT (Phospho-Ser473) 6 1 3 10 AKT (Phospho-Thr308) 7 1 3 11 FAK (Phospho-Tyr861) 8 1 3 12 BAD (Phospho-Ser112) 9 1 3 13 BAD (Phospho-Ser136) 10 1 3 14 BAD (Phospho-Ser155) 11 1 3 15 VEGFR2 (Phospho-Tyr1175) 12 1 3 16 VEGFR2 (Phospho-Tyr1214) 13 1 3 17 VEGFR2 (Phospho-Tyr951) 14 1 3 18 Paxillin (Phospho-Tyr118) 15 1 3 19 Src (Phospho-Tyr418) 16 1 3 20 FKHR (Phospho-Ser256) 17 1 3 21 FAK (Phospho-Tyr925) 18 1 3 22 AKT2 (Phospho-Ser474) 19 1 3 23 FKHR (Phospho-Ser319) 20 1 3 24 MKK3 (Phospho-Ser189 21 1 3 25 Src (Phospho-Tyr529) 22 1 3 26 eNOS (Phospho-Ser1177) 23 1 3 27 MEK1 (Phospho-Ser221) 24 1 3 28 HSP27 (Phospho-Ser15) 25 1 3 29 Paxillin (Phospho-Tyr31) 26 1 3 30 Raf1 (Phospho-Ser338) 27 1 3 31 MEK1 (Phospho-Ser217) 28 1 3 32 eNOS (Phospho-Thr495) 29 2 3 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 3 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 3 3 HSP27 (Phospho-Ser78) 32 2 3 4 HSP27 (Phospho-Ser82) 33 2 3 5 P38 MAPK (Phospho-Thr180) 34 2 3 6 P38 MAPK (Phospho-Tyr182) 35 2 3 7 MEK1 (Phospho-Thr291) 36 2 3 8 PKC delta (Phospho-Ser645) 37 2 3 9 PKC theta (Phospho-Ser676) 38 2 3 10 HSP90B (Phospho-Ser254) 39 2 3 11 PKC zeta (Phospho-Thr410) 40 2 3 12 Shc (Phospho-Tyr349) 41 2 3 13 Shc (Phospho-Tyr427) 42 2 3 14 Elk-1 (Ab-383) 43 2 3 15 Raf1 (Ab-259) 44 2 3 16 MEK-2 (Ab-394) 45 2 3 17 Elk1 (Ab-389) 46 2 3 18 Elk1 (Ab-417) 47 2 3 19 AKT (Ab-473) 48 2 3 20 AKT (Ab-308) 49 2 3 21 BAD (Ab-112) 50 2 3 22 BAD (Ab-136) 51 2 3 23 BAD (Ab-155) 52 2 3 24 FAK (Ab-861) 53 2 3 25 VEGFR2 (Ab-1175) 54 2 3 26 VEGFR2 (Ab-1214) 55 2 3 27 VEGFR2 (Ab-951) 56 2 3 28 Paxillin (Ab-118) 57 2 3 29 Src (Ab-418) 58 2 3 30 MKK3 (Ab-189) 59 2 3 31 FKHR (Ab-256) 60 2 3 32 HSP27 (Ab-15) 61 3 3 1 FAK (Ab-925) 62 3 3 2 AKT2 (Ab-474) 63 3 3 3 FKHR (Ab-319) 64 3 3 4 Src (Ab-529) 65 3 3 5 eNOS (Ab-1177) 66 3 3 6 MEK1 (Ab-221) 67 3 3 7 Paxillin (Ab-31) 68 3 3 8 Raf1 (Ab-338) 69 3 3 9 MEK1 (Ab-217) 70 3 3 10 FAK (Ab-397) 71 3 3 11 eNOS (Ab-495) 72 3 3 12 p44/42 MAP Kinase (Ab-202) 73 3 3 13 p44/42 MAP Kinase (Ab-204) 74 3 3 14 HSP27 (Ab-78) 75 3 3 15 HSP27 (Ab-82) 76 3 3 16 P38 MAPK (Ab-182) 77 3 3 17 MEK1 (Ab-291) 78 3 3 18 PKC delta (Ab-645) 79 3 3 19 PKC theta (Ab-676) 80 3 3 20 HSP90B (Ab-254) 81 3 3 21 PKC zeta (Ab-410) 82 3 3 22 Shc (Ab-349) 83 3 3 23 MKK6 (Phospho-Ser207) 84 3 3 24 FAK (Phospho-Tyr397) 85 3 3 25 MAPKAPK2 (Phospho-Thr334) 86 3 3 26 c-PLA2 (Phospho-Ser505) 87 3 3 27 AKT1 (Phospho-Thr450) 88 3 3 28 FAK (Phospho-Tyr576) 89 3 3 29 FAK (Phospho-Tyr407) 90 3 3 30 Raf1 (Phospho-Tyr341) 91 3 3 31 Raf1(Phospho-Ser621) 92 3 3 32 VEGFR-1 (Phospho-Tyr1333) 93 4 3 1 VEGFR2 (Phospho-Tyr1059) 94 4 3 2 AKT1 (Phospho-Thr72) 95 4 3 3 MAPKAPK-2 (Phospho-Thr222) 96 4 3 4 MEK1 (Phospho-Ser298) 97 4 3 5 MEK1 (Phospho-Thr286) 98 4 3 6 PKC alpha/beta II (Phospho-Thr638) 99 4 3 7 PKC delta (Phospho-Thr505) 100 4 3 8 PKC theta (Phospho-Thr538) 101 4 3 9 Ras-GRF1 (Phospho-Ser916) 102 4 3 10 p38 MAPK (Phospho-Tyr322) 103 4 3 11 PKC alpha (Phospho-Tyr657) 104 4 3 12 PKC epsilon (Phospho-Ser729) 105 4 3 13 PKC zeta (Phospho-Thr560) 106 4 3 14 AKT (Phospho-Tyr326) 107 4 3 15 BAD (Phospho-Ser91/128) 108 4 3 16 BAD (Phospho-Ser134) 109 4 3 17 Caspase 9 (Phospho-Tyr153) 110 4 3 18 Caspase 9 (Phospho-Ser144) 111 4 3 19 Caspase 9 (Phospho-Ser196) 112 4 3 20 ERK3 (Phospho-Ser189) 113 4 3 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 3 22 FAK (Phospho-Ser910) 115 4 3 23 GTPase activating protein (Phopspho-Ser387) 116 4 3 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 3 25 RapGEF1 (Phospho-Tyr504) 118 4 3 26 eNOS (Phospho-Ser615) 119 4 3 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 3 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 3 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 3 30 PKC beta/PKCB (Phospho-Ser661) 123 4 3 31 Src (Phospho-Ser75) 124 4 3 32 Src (Phospho-Tyr216) 125 5 3 1 GRB2 (Phospho-Ser159) 126 5 3 2 MAPKAPK2 (Phospho-Ser272) 127 5 3 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 3 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 3 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 3 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 3 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 3 8 VEGF R2 (Phospho-Tyr1054) 133 5 3 9 MKK6 (Ab-207) 134 5 3 10 MAPKAPK2 (Ab-334) 135 5 3 11 Shc (Ab-427) 136 5 3 12 c-PLA2 (Ab-505) 137 5 3 13 AKT1 (Ab-450) 138 5 3 14 FAK (Ab-576) 139 5 3 15 FAK (Ab-407) 140 5 3 16 Raf1 (Ab-341) 141 5 3 17 Raf1 (Ab-621) 142 5 3 18 VEGFR-1 (Ab-1333) 143 5 3 19 VEGFR2 (Ab-1059) 144 5 3 20 AKT1 (Ab-72) 145 5 3 21 MEK1 (Ab-298) 146 5 3 22 MEK1 (Ab286) 147 5 3 23 PKC alpha/beta II (Ab-638) 148 5 3 24 PKC delta (Ab-505) 149 5 3 25 PKC theta (Ab-538) 150 5 3 26 Ras-GRF1 (Ab-916) 151 5 3 27 p38 MAPK (Ab-322) 152 5 3 28 PKC alpha (Ab-657) 153 5 3 29 PKC epsilon (Ab-729) 154 5 3 30 PKC zeta (Ab-560) 155 5 3 31 AKT (Ab-326) 156 5 3 32 BAD (Ab-91/128) 157 6 3 1 BAD (Ab-134) 158 6 3 2 Caspase 9 (Ab-153) 159 6 3 3 Caspase 9 (Ab-144) 160 6 3 4 Caspase 9 (Ab-196) 161 6 3 5 ERK3 (Ab-189) 162 6 3 6 FAK (Ab-910) 163 6 3 7 GTPase activating protein (Ab-387) 164 6 3 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 3 9 eNOS (Ab-615) 166 6 3 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 3 11 AKT1/2/3 (Ab-315) 168 6 3 12 MKK3/MAP2K3 (Ab-222) 169 6 3 13 PKC beta/PKCB (Ab-661) 170 6 3 14 Raf1 (Ab-289) 171 6 3 15 Src (Ab-75) 172 6 3 16 GRB2 (Ab-159) 173 6 3 17 MAPKAPK2 (Ab-272) 174 6 3 18 PLC-gamma 1 (Ab-771) 175 6 3 19 PLC-gamma 1 (Ab-783) 176 6 3 20 PLC-gamma 2 (Ab-753) 177 6 3 21 PLC-gamma 2 (Ab-1217) 178 6 3 22 VEGF R2 (Ab-1054) 179 6 3 23 ERK1/2 (N-term) 180 6 3 24 HSP90A (C-term) 181 6 3 25 RAS(p21 H and K) (inter) 182 6 3 26 IP3KA (C-term) 183 6 3 27 IP3KC (inter) 184 6 3 28 RASE (inter) 185 6 3 29 Beta actin A 6 3 30 GAPDH G 6 3 31 Negative Control N 6 3 32 Positive Marker P 1 4 1 Positive Marker P 1 4 2 Empty E 1 4 3 Empty E 1 4 4 Elk-1 (Phospho-Ser383) 1 1 4 5 Raf1 (Phospho-Ser259) 2 1 4 6 MEK-2 (Phospho-Thr394) 3 1 4 7 Elk1 (Phospho-Ser389) 4 1 4 8 Elk1 (Phospho-Thr417) 5 1 4 9 AKT (Phospho-Ser473) 6 1 4 10 AKT (Phospho-Thr308) 7 1 4 11 FAK (Phospho-Tyr861) 8 1 4 12 BAD (Phospho-Ser112) 9 1 4 13 BAD (Phospho-Ser136) 10 1 4 14 BAD (Phospho-Ser155) 11 1 4 15 VEGFR2 (Phospho-Tyr1175) 12 1 4 16 VEGFR2 (Phospho-Tyr1214) 13 1 4 17 VEGFR2 (Phospho-Tyr951) 14 1 4 18 Paxillin (Phospho-Tyr118) 15 1 4 19 Src (Phospho-Tyr418) 16 1 4 20 FKHR (Phospho-Ser256) 17 1 4 21 FAK (Phospho-Tyr925) 18 1 4 22 AKT2 (Phospho-Ser474) 19 1 4 23 FKHR (Phospho-Ser319) 20 1 4 24 MKK3 (Phospho-Ser189 21 1 4 25 Src (Phospho-Tyr529) 22 1 4 26 eNOS (Phospho-Ser1177) 23 1 4 27 MEK1 (Phospho-Ser221) 24 1 4 28 HSP27 (Phospho-Ser15) 25 1 4 29 Paxillin (Phospho-Tyr31) 26 1 4 30 Raf1 (Phospho-Ser338) 27 1 4 31 MEK1 (Phospho-Ser217) 28 1 4 32 eNOS (Phospho-Thr495) 29 2 4 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 4 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 4 3 HSP27 (Phospho-Ser78) 32 2 4 4 HSP27 (Phospho-Ser82) 33 2 4 5 P38 MAPK (Phospho-Thr180) 34 2 4 6 P38 MAPK (Phospho-Tyr182) 35 2 4 7 MEK1 (Phospho-Thr291) 36 2 4 8 PKC delta (Phospho-Ser645) 37 2 4 9 PKC theta (Phospho-Ser676) 38 2 4 10 HSP90B (Phospho-Ser254) 39 2 4 11 PKC zeta (Phospho-Thr410) 40 2 4 12 Shc (Phospho-Tyr349) 41 2 4 13 Shc (Phospho-Tyr427) 42 2 4 14 Elk-1 (Ab-383) 43 2 4 15 Raf1 (Ab-259) 44 2 4 16 MEK-2 (Ab-394) 45 2 4 17 Elk1 (Ab-389) 46 2 4 18 Elk1 (Ab-417) 47 2 4 19 AKT (Ab-473) 48 2 4 20 AKT (Ab-308) 49 2 4 21 BAD (Ab-112) 50 2 4 22 BAD (Ab-136) 51 2 4 23 BAD (Ab-155) 52 2 4 24 FAK (Ab-861) 53 2 4 25 VEGFR2 (Ab-1175) 54 2 4 26 VEGFR2 (Ab-1214) 55 2 4 27 VEGFR2 (Ab-951) 56 2 4 28 Paxillin (Ab-118) 57 2 4 29 Src (Ab-418) 58 2 4 30 MKK3 (Ab-189) 59 2 4 31 FKHR (Ab-256) 60 2 4 32 HSP27 (Ab-15) 61 3 4 1 FAK (Ab-925) 62 3 4 2 AKT2 (Ab-474) 63 3 4 3 FKHR (Ab-319) 64 3 4 4 Src (Ab-529) 65 3 4 5 eNOS (Ab-1177) 66 3 4 6 MEK1 (Ab-221) 67 3 4 7 Paxillin (Ab-31) 68 3 4 8 Raf1 (Ab-338) 69 3 4 9 MEK1 (Ab-217) 70 3 4 10 FAK (Ab-397) 71 3 4 11 eNOS (Ab-495) 72 3 4 12 p44/42 MAP Kinase (Ab-202) 73 3 4 13 p44/42 MAP Kinase (Ab-204) 74 3 4 14 HSP27 (Ab-78) 75 3 4 15 HSP27 (Ab-82) 76 3 4 16 P38 MAPK (Ab-182) 77 3 4 17 MEK1 (Ab-291) 78 3 4 18 PKC delta (Ab-645) 79 3 4 19 PKC theta (Ab-676) 80 3 4 20 HSP90B (Ab-254) 81 3 4 21 PKC zeta (Ab-410) 82 3 4 22 Shc (Ab-349) 83 3 4 23 MKK6 (Phospho-Ser207) 84 3 4 24 FAK (Phospho-Tyr397) 85 3 4 25 MAPKAPK2 (Phospho-Thr334) 86 3 4 26 c-PLA2 (Phospho-Ser505) 87 3 4 27 AKT1 (Phospho-Thr450) 88 3 4 28 FAK (Phospho-Tyr576) 89 3 4 29 FAK (Phospho-Tyr407) 90 3 4 30 Raf1 (Phospho-Tyr341) 91 3 4 31 Raf1(Phospho-Ser621) 92 3 4 32 VEGFR-1 (Phospho-Tyr1333) 93 4 4 1 VEGFR2 (Phospho-Tyr1059) 94 4 4 2 AKT1 (Phospho-Thr72) 95 4 4 3 MAPKAPK-2 (Phospho-Thr222) 96 4 4 4 MEK1 (Phospho-Ser298) 97 4 4 5 MEK1 (Phospho-Thr286) 98 4 4 6 PKC alpha/beta II (Phospho-Thr638) 99 4 4 7 PKC delta (Phospho-Thr505) 100 4 4 8 PKC theta (Phospho-Thr538) 101 4 4 9 Ras-GRF1 (Phospho-Ser916) 102 4 4 10 p38 MAPK (Phospho-Tyr322) 103 4 4 11 PKC alpha (Phospho-Tyr657) 104 4 4 12 PKC epsilon (Phospho-Ser729) 105 4 4 13 PKC zeta (Phospho-Thr560) 106 4 4 14 AKT (Phospho-Tyr326) 107 4 4 15 BAD (Phospho-Ser91/128) 108 4 4 16 BAD (Phospho-Ser134) 109 4 4 17 Caspase 9 (Phospho-Tyr153) 110 4 4 18 Caspase 9 (Phospho-Ser144) 111 4 4 19 Caspase 9 (Phospho-Ser196) 112 4 4 20 ERK3 (Phospho-Ser189) 113 4 4 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 4 22 FAK (Phospho-Ser910) 115 4 4 23 GTPase activating protein (Phopspho-Ser387) 116 4 4 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 4 25 RapGEF1 (Phospho-Tyr504) 118 4 4 26 eNOS (Phospho-Ser615) 119 4 4 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 4 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 4 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 4 30 PKC beta/PKCB (Phospho-Ser661) 123 4 4 31 Src (Phospho-Ser75) 124 4 4 32 Src (Phospho-Tyr216) 125 5 4 1 GRB2 (Phospho-Ser159) 126 5 4 2 MAPKAPK2 (Phospho-Ser272) 127 5 4 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 4 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 4 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 4 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 4 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 4 8 VEGF R2 (Phospho-Tyr1054) 133 5 4 9 MKK6 (Ab-207) 134 5 4 10 MAPKAPK2 (Ab-334) 135 5 4 11 Shc (Ab-427) 136 5 4 12 c-PLA2 (Ab-505) 137 5 4 13 AKT1 (Ab-450) 138 5 4 14 FAK (Ab-576) 139 5 4 15 FAK (Ab-407) 140 5 4 16 Raf1 (Ab-341) 141 5 4 17 Raf1 (Ab-621) 142 5 4 18 VEGFR-1 (Ab-1333) 143 5 4 19 VEGFR2 (Ab-1059) 144 5 4 20 AKT1 (Ab-72) 145 5 4 21 MEK1 (Ab-298) 146 5 4 22 MEK1 (Ab286) 147 5 4 23 PKC alpha/beta II (Ab-638) 148 5 4 24 PKC delta (Ab-505) 149 5 4 25 PKC theta (Ab-538) 150 5 4 26 Ras-GRF1 (Ab-916) 151 5 4 27 p38 MAPK (Ab-322) 152 5 4 28 PKC alpha (Ab-657) 153 5 4 29 PKC epsilon (Ab-729) 154 5 4 30 PKC zeta (Ab-560) 155 5 4 31 AKT (Ab-326) 156 5 4 32 BAD (Ab-91/128) 157 6 4 1 BAD (Ab-134) 158 6 4 2 Caspase 9 (Ab-153) 159 6 4 3 Caspase 9 (Ab-144) 160 6 4 4 Caspase 9 (Ab-196) 161 6 4 5 ERK3 (Ab-189) 162 6 4 6 FAK (Ab-910) 163 6 4 7 GTPase activating protein (Ab-387) 164 6 4 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 4 9 eNOS (Ab-615) 166 6 4 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 4 11 AKT1/2/3 (Ab-315) 168 6 4 12 MKK3/MAP2K3 (Ab-222) 169 6 4 13 PKC beta/PKCB (Ab-661) 170 6 4 14 Raf1 (Ab-289) 171 6 4 15 Src (Ab-75) 172 6 4 16 GRB2 (Ab-159) 173 6 4 17 MAPKAPK2 (Ab-272) 174 6 4 18 PLC-gamma 1 (Ab-771) 175 6 4 19 PLC-gamma 1 (Ab-783) 176 6 4 20 PLC-gamma 2 (Ab-753) 177 6 4 21 PLC-gamma 2 (Ab-1217) 178 6 4 22 VEGF R2 (Ab-1054) 179 6 4 23 ERK1/2 (N-term) 180 6 4 24 HSP90A (C-term) 181 6 4 25 RAS(p21 H and K) (inter) 182 6 4 26 IP3KA (C-term) 183 6 4 27 IP3KC (inter) 184 6 4 28 RASE (inter) 185 6 4 29 Beta actin A 6 4 30 GAPDH G 6 4 31 Negative Control N 6 4 32 Positive Marker P 1 5 1 Positive Marker P 1 5 2 Empty E 1 5 3 Empty E 1 5 4 Elk-1 (Phospho-Ser383) 1 1 5 5 Raf1 (Phospho-Ser259) 2 1 5 6 MEK-2 (Phospho-Thr394) 3 1 5 7 Elk1 (Phospho-Ser389) 4 1 5 8 Elk1 (Phospho-Thr417) 5 1 5 9 AKT (Phospho-Ser473) 6 1 5 10 AKT (Phospho-Thr308) 7 1 5 11 FAK (Phospho-Tyr861) 8 1 5 12 BAD (Phospho-Ser112) 9 1 5 13 BAD (Phospho-Ser136) 10 1 5 14 BAD (Phospho-Ser155) 11 1 5 15 VEGFR2 (Phospho-Tyr1175) 12 1 5 16 VEGFR2 (Phospho-Tyr1214) 13 1 5 17 VEGFR2 (Phospho-Tyr951) 14 1 5 18 Paxillin (Phospho-Tyr118) 15 1 5 19 Src (Phospho-Tyr418) 16 1 5 20 FKHR (Phospho-Ser256) 17 1 5 21 FAK (Phospho-Tyr925) 18 1 5 22 AKT2 (Phospho-Ser474) 19 1 5 23 FKHR (Phospho-Ser319) 20 1 5 24 MKK3 (Phospho-Ser189 21 1 5 25 Src (Phospho-Tyr529) 22 1 5 26 eNOS (Phospho-Ser1177) 23 1 5 27 MEK1 (Phospho-Ser221) 24 1 5 28 HSP27 (Phospho-Ser15) 25 1 5 29 Paxillin (Phospho-Tyr31) 26 1 5 30 Raf1 (Phospho-Ser338) 27 1 5 31 MEK1 (Phospho-Ser217) 28 1 5 32 eNOS (Phospho-Thr495) 29 2 5 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 5 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 5 3 HSP27 (Phospho-Ser78) 32 2 5 4 HSP27 (Phospho-Ser82) 33 2 5 5 P38 MAPK (Phospho-Thr180) 34 2 5 6 P38 MAPK (Phospho-Tyr182) 35 2 5 7 MEK1 (Phospho-Thr291) 36 2 5 8 PKC delta (Phospho-Ser645) 37 2 5 9 PKC theta (Phospho-Ser676) 38 2 5 10 HSP90B (Phospho-Ser254) 39 2 5 11 PKC zeta (Phospho-Thr410) 40 2 5 12 Shc (Phospho-Tyr349) 41 2 5 13 Shc (Phospho-Tyr427) 42 2 5 14 Elk-1 (Ab-383) 43 2 5 15 Raf1 (Ab-259) 44 2 5 16 MEK-2 (Ab-394) 45 2 5 17 Elk1 (Ab-389) 46 2 5 18 Elk1 (Ab-417) 47 2 5 19 AKT (Ab-473) 48 2 5 20 AKT (Ab-308) 49 2 5 21 BAD (Ab-112) 50 2 5 22 BAD (Ab-136) 51 2 5 23 BAD (Ab-155) 52 2 5 24 FAK (Ab-861) 53 2 5 25 VEGFR2 (Ab-1175) 54 2 5 26 VEGFR2 (Ab-1214) 55 2 5 27 VEGFR2 (Ab-951) 56 2 5 28 Paxillin (Ab-118) 57 2 5 29 Src (Ab-418) 58 2 5 30 MKK3 (Ab-189) 59 2 5 31 FKHR (Ab-256) 60 2 5 32 HSP27 (Ab-15) 61 3 5 1 FAK (Ab-925) 62 3 5 2 AKT2 (Ab-474) 63 3 5 3 FKHR (Ab-319) 64 3 5 4 Src (Ab-529) 65 3 5 5 eNOS (Ab-1177) 66 3 5 6 MEK1 (Ab-221) 67 3 5 7 Paxillin (Ab-31) 68 3 5 8 Raf1 (Ab-338) 69 3 5 9 MEK1 (Ab-217) 70 3 5 10 FAK (Ab-397) 71 3 5 11 eNOS (Ab-495) 72 3 5 12 p44/42 MAP Kinase (Ab-202) 73 3 5 13 p44/42 MAP Kinase (Ab-204) 74 3 5 14 HSP27 (Ab-78) 75 3 5 15 HSP27 (Ab-82) 76 3 5 16 P38 MAPK (Ab-182) 77 3 5 17 MEK1 (Ab-291) 78 3 5 18 PKC delta (Ab-645) 79 3 5 19 PKC theta (Ab-676) 80 3 5 20 HSP90B (Ab-254) 81 3 5 21 PKC zeta (Ab-410) 82 3 5 22 Shc (Ab-349) 83 3 5 23 MKK6 (Phospho-Ser207) 84 3 5 24 FAK (Phospho-Tyr397) 85 3 5 25 MAPKAPK2 (Phospho-Thr334) 86 3 5 26 c-PLA2 (Phospho-Ser505) 87 3 5 27 AKT1 (Phospho-Thr450) 88 3 5 28 FAK (Phospho-Tyr576) 89 3 5 29 FAK (Phospho-Tyr407) 90 3 5 30 Raf1 (Phospho-Tyr341) 91 3 5 31 Raf1(Phospho-Ser621) 92 3 5 32 VEGFR-1 (Phospho-Tyr1333) 93 4 5 1 VEGFR2 (Phospho-Tyr1059) 94 4 5 2 AKT1 (Phospho-Thr72) 95 4 5 3 MAPKAPK-2 (Phospho-Thr222) 96 4 5 4 MEK1 (Phospho-Ser298) 97 4 5 5 MEK1 (Phospho-Thr286) 98 4 5 6 PKC alpha/beta II (Phospho-Thr638) 99 4 5 7 PKC delta (Phospho-Thr505) 100 4 5 8 PKC theta (Phospho-Thr538) 101 4 5 9 Ras-GRF1 (Phospho-Ser916) 102 4 5 10 p38 MAPK (Phospho-Tyr322) 103 4 5 11 PKC alpha (Phospho-Tyr657) 104 4 5 12 PKC epsilon (Phospho-Ser729) 105 4 5 13 PKC zeta (Phospho-Thr560) 106 4 5 14 AKT (Phospho-Tyr326) 107 4 5 15 BAD (Phospho-Ser91/128) 108 4 5 16 BAD (Phospho-Ser134) 109 4 5 17 Caspase 9 (Phospho-Tyr153) 110 4 5 18 Caspase 9 (Phospho-Ser144) 111 4 5 19 Caspase 9 (Phospho-Ser196) 112 4 5 20 ERK3 (Phospho-Ser189) 113 4 5 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 5 22 FAK (Phospho-Ser910) 115 4 5 23 GTPase activating protein (Phopspho-Ser387) 116 4 5 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 5 25 RapGEF1 (Phospho-Tyr504) 118 4 5 26 eNOS (Phospho-Ser615) 119 4 5 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 5 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 5 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 5 30 PKC beta/PKCB (Phospho-Ser661) 123 4 5 31 Src (Phospho-Ser75) 124 4 5 32 Src (Phospho-Tyr216) 125 5 5 1 GRB2 (Phospho-Ser159) 126 5 5 2 MAPKAPK2 (Phospho-Ser272) 127 5 5 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 5 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 5 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 5 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 5 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 5 8 VEGF R2 (Phospho-Tyr1054) 133 5 5 9 MKK6 (Ab-207) 134 5 5 10 MAPKAPK2 (Ab-334) 135 5 5 11 Shc (Ab-427) 136 5 5 12 c-PLA2 (Ab-505) 137 5 5 13 AKT1 (Ab-450) 138 5 5 14 FAK (Ab-576) 139 5 5 15 FAK (Ab-407) 140 5 5 16 Raf1 (Ab-341) 141 5 5 17 Raf1 (Ab-621) 142 5 5 18 VEGFR-1 (Ab-1333) 143 5 5 19 VEGFR2 (Ab-1059) 144 5 5 20 AKT1 (Ab-72) 145 5 5 21 MEK1 (Ab-298) 146 5 5 22 MEK1 (Ab286) 147 5 5 23 PKC alpha/beta II (Ab-638) 148 5 5 24 PKC delta (Ab-505) 149 5 5 25 PKC theta (Ab-538) 150 5 5 26 Ras-GRF1 (Ab-916) 151 5 5 27 p38 MAPK (Ab-322) 152 5 5 28 PKC alpha (Ab-657) 153 5 5 29 PKC epsilon (Ab-729) 154 5 5 30 PKC zeta (Ab-560) 155 5 5 31 AKT (Ab-326) 156 5 5 32 BAD (Ab-91/128) 157 6 5 1 BAD (Ab-134) 158 6 5 2 Caspase 9 (Ab-153) 159 6 5 3 Caspase 9 (Ab-144) 160 6 5 4 Caspase 9 (Ab-196) 161 6 5 5 ERK3 (Ab-189) 162 6 5 6 FAK (Ab-910) 163 6 5 7 GTPase activating protein (Ab-387) 164 6 5 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 5 9 eNOS (Ab-615) 166 6 5 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 5 11 AKT1/2/3 (Ab-315) 168 6 5 12 MKK3/MAP2K3 (Ab-222) 169 6 5 13 PKC beta/PKCB (Ab-661) 170 6 5 14 Raf1 (Ab-289) 171 6 5 15 Src (Ab-75) 172 6 5 16 GRB2 (Ab-159) 173 6 5 17 MAPKAPK2 (Ab-272) 174 6 5 18 PLC-gamma 1 (Ab-771) 175 6 5 19 PLC-gamma 1 (Ab-783) 176 6 5 20 PLC-gamma 2 (Ab-753) 177 6 5 21 PLC-gamma 2 (Ab-1217) 178 6 5 22 VEGF R2 (Ab-1054) 179 6 5 23 ERK1/2 (N-term) 180 6 5 24 HSP90A (C-term) 181 6 5 25 RAS(p21 H and K) (inter) 182 6 5 26 IP3KA (C-term) 183 6 5 27 IP3KC (inter) 184 6 5 28 RASE (inter) 185 6 5 29 Beta actin A 6 5 30 GAPDH G 6 5 31 Negative Control N 6 5 32 Positive Marker P 1 6 1 Positive Marker P 1 6 2 Empty E 1 6 3 Empty E 1 6 4 Elk-1 (Phospho-Ser383) 1 1 6 5 Raf1 (Phospho-Ser259) 2 1 6 6 MEK-2 (Phospho-Thr394) 3 1 6 7 Elk1 (Phospho-Ser389) 4 1 6 8 Elk1 (Phospho-Thr417) 5 1 6 9 AKT (Phospho-Ser473) 6 1 6 10 AKT (Phospho-Thr308) 7 1 6 11 FAK (Phospho-Tyr861) 8 1 6 12 BAD (Phospho-Ser112) 9 1 6 13 BAD (Phospho-Ser136) 10 1 6 14 BAD (Phospho-Ser155) 11 1 6 15 VEGFR2 (Phospho-Tyr1175) 12 1 6 16 VEGFR2 (Phospho-Tyr1214) 13 1 6 17 VEGFR2 (Phospho-Tyr951) 14 1 6 18 Paxillin (Phospho-Tyr118) 15 1 6 19 Src (Phospho-Tyr418) 16 1 6 20 FKHR (Phospho-Ser256) 17 1 6 21 FAK (Phospho-Tyr925) 18 1 6 22 AKT2 (Phospho-Ser474) 19 1 6 23 FKHR (Phospho-Ser319) 20 1 6 24 MKK3 (Phospho-Ser189 21 1 6 25 Src (Phospho-Tyr529) 22 1 6 26 eNOS (Phospho-Ser1177) 23 1 6 27 MEK1 (Phospho-Ser221) 24 1 6 28 HSP27 (Phospho-Ser15) 25 1 6 29 Paxillin (Phospho-Tyr31) 26 1 6 30 Raf1 (Phospho-Ser338) 27 1 6 31 MEK1 (Phospho-Ser217) 28 1 6 32 eNOS (Phospho-Thr495) 29 2 6 1 p44/42 MAP Kinase (Phospho-Thr202) 30 2 6 2 p44/42 MAP Kinase (Phospho-Tyr204) 31 2 6 3 HSP27 (Phospho-Ser78) 32 2 6 4 HSP27 (Phospho-Ser82) 33 2 6 5 P38 MAPK (Phospho-Thr180) 34 2 6 6 P38 MAPK (Phospho-Tyr182) 35 2 6 7 MEK1 (Phospho-Thr291) 36 2 6 8 PKC delta (Phospho-Ser645) 37 2 6 9 PKC theta (Phospho-Ser676) 38 2 6 10 HSP90B (Phospho-Ser254) 39 2 6 11 PKC zeta (Phospho-Thr410) 40 2 6 12 Shc (Phospho-Tyr349) 41 2 6 13 Shc (Phospho-Tyr427) 42 2 6 14 Elk-1 (Ab-383) 43 2 6 15 Raf1 (Ab-259) 44 2 6 16 MEK-2 (Ab-394) 45 2 6 17 Elk1 (Ab-389) 46 2 6 18 Elk1 (Ab-417) 47 2 6 19 AKT (Ab-473) 48 2 6 20 AKT (Ab-308) 49 2 6 21 BAD (Ab-112) 50 2 6 22 BAD (Ab-136) 51 2 6 23 BAD (Ab-155) 52 2 6 24 FAK (Ab-861) 53 2 6 25 VEGFR2 (Ab-1175) 54 2 6 26 VEGFR2 (Ab-1214) 55 2 6 27 VEGFR2 (Ab-951) 56 2 6 28 Paxillin (Ab-118) 57 2 6 29 Src (Ab-418) 58 2 6 30 MKK3 (Ab-189) 59 2 6 31 FKHR (Ab-256) 60 2 6 32 HSP27 (Ab-15) 61 3 6 1 FAK (Ab-925) 62 3 6 2 AKT2 (Ab-474) 63 3 6 3 FKHR (Ab-319) 64 3 6 4 Src (Ab-529) 65 3 6 5 eNOS (Ab-1177) 66 3 6 6 MEK1 (Ab-221) 67 3 6 7 Paxillin (Ab-31) 68 3 6 8 Raf1 (Ab-338) 69 3 6 9 MEK1 (Ab-217) 70 3 6 10 FAK (Ab-397) 71 3 6 11 eNOS (Ab-495) 72 3 6 12 p44/42 MAP Kinase (Ab-202) 73 3 6 13 p44/42 MAP Kinase (Ab-204) 74 3 6 14 HSP27 (Ab-78) 75 3 6 15 HSP27 (Ab-82) 76 3 6 16 P38 MAPK (Ab-182) 77 3 6 17 MEK1 (Ab-291) 78 3 6 18 PKC delta (Ab-645) 79 3 6 19 PKC theta (Ab-676) 80 3 6 20 HSP90B (Ab-254) 81 3 6 21 PKC zeta (Ab-410) 82 3 6 22 Shc (Ab-349) 83 3 6 23 MKK6 (Phospho-Ser207) 84 3 6 24 FAK (Phospho-Tyr397) 85 3 6 25 MAPKAPK2 (Phospho-Thr334) 86 3 6 26 c-PLA2 (Phospho-Ser505) 87 3 6 27 AKT1 (Phospho-Thr450) 88 3 6 28 FAK (Phospho-Tyr576) 89 3 6 29 FAK (Phospho-Tyr407) 90 3 6 30 Raf1 (Phospho-Tyr341) 91 3 6 31 Raf1(Phospho-Ser621) 92 3 6 32 VEGFR-1 (Phospho-Tyr1333) 93 4 6 1 VEGFR2 (Phospho-Tyr1059) 94 4 6 2 AKT1 (Phospho-Thr72) 95 4 6 3 MAPKAPK-2 (Phospho-Thr222) 96 4 6 4 MEK1 (Phospho-Ser298) 97 4 6 5 MEK1 (Phospho-Thr286) 98 4 6 6 PKC alpha/beta II (Phospho-Thr638) 99 4 6 7 PKC delta (Phospho-Thr505) 100 4 6 8 PKC theta (Phospho-Thr538) 101 4 6 9 Ras-GRF1 (Phospho-Ser916) 102 4 6 10 p38 MAPK (Phospho-Tyr322) 103 4 6 11 PKC alpha (Phospho-Tyr657) 104 4 6 12 PKC epsilon (Phospho-Ser729) 105 4 6 13 PKC zeta (Phospho-Thr560) 106 4 6 14 AKT (Phospho-Tyr326) 107 4 6 15 BAD (Phospho-Ser91/128) 108 4 6 16 BAD (Phospho-Ser134) 109 4 6 17 Caspase 9 (Phospho-Tyr153) 110 4 6 18 Caspase 9 (Phospho-Ser144) 111 4 6 19 Caspase 9 (Phospho-Ser196) 112 4 6 20 ERK3 (Phospho-Ser189) 113 4 6 21 ERK8 (Phospho-Thr175/Tyr177) 114 4 6 22 FAK (Phospho-Ser910) 115 4 6 23 GTPase activating protein (Phopspho-Ser387) 116 4 6 24 Hsp90 co-chaperone Cdc37 (Phospho-Ser13) 117 4 6 25 RapGEF1 (Phospho-Tyr504) 118 4 6 26 eNOS (Phospho-Ser615) 119 4 6 27 PI3-kinase p85-subunit alpha/gamma (Phospho-Tyr467/Tyr199) 120 4 6 28 PI3-kinase p85-alpha (Phospho-Tyr607) 121 4 6 29 MKK3/MAP2K3 (Phospho-Thr222) 122 4 6 30 PKC beta/PKCB (Phospho-Ser661) 123 4 6 31 Src (Phospho-Ser75) 124 4 6 32 Src (Phospho-Tyr216) 125 5 6 1 GRB2 (Phospho-Ser159) 126 5 6 2 MAPKAPK2 (Phospho-Ser272) 127 5 6 3 PLC-gamma 1 (Phospho-Tyr771) 128 5 6 4 PLC-gamma 1 (Phospho-Tyr783) 129 5 6 5 PLC-gamma 2 (Phospho-Tyr753) 130 5 6 6 PLC-gamma 1 (Phospho-Tyr1253) 131 5 6 7 PLC-gamma 2 (Phospho-Tyr1217) 132 5 6 8 VEGF R2 (Phospho-Tyr1054) 133 5 6 9 MKK6 (Ab-207) 134 5 6 10 MAPKAPK2 (Ab-334) 135 5 6 11 Shc (Ab-427) 136 5 6 12 c-PLA2 (Ab-505) 137 5 6 13 AKT1 (Ab-450) 138 5 6 14 FAK (Ab-576) 139 5 6 15 FAK (Ab-407) 140 5 6 16 Raf1 (Ab-341) 141 5 6 17 Raf1 (Ab-621) 142 5 6 18 VEGFR-1 (Ab-1333) 143 5 6 19 VEGFR2 (Ab-1059) 144 5 6 20 AKT1 (Ab-72) 145 5 6 21 MEK1 (Ab-298) 146 5 6 22 MEK1 (Ab286) 147 5 6 23 PKC alpha/beta II (Ab-638) 148 5 6 24 PKC delta (Ab-505) 149 5 6 25 PKC theta (Ab-538) 150 5 6 26 Ras-GRF1 (Ab-916) 151 5 6 27 p38 MAPK (Ab-322) 152 5 6 28 PKC alpha (Ab-657) 153 5 6 29 PKC epsilon (Ab-729) 154 5 6 30 PKC zeta (Ab-560) 155 5 6 31 AKT (Ab-326) 156 5 6 32 BAD (Ab-91/128) 157 6 6 1 BAD (Ab-134) 158 6 6 2 Caspase 9 (Ab-153) 159 6 6 3 Caspase 9 (Ab-144) 160 6 6 4 Caspase 9 (Ab-196) 161 6 6 5 ERK3 (Ab-189) 162 6 6 6 FAK (Ab-910) 163 6 6 7 GTPase activating protein (Ab-387) 164 6 6 8 Hsp90 co-chaperone Cdc37 (Ab-13) 165 6 6 9 eNOS (Ab-615) 166 6 6 10 PI3-kinase p85-subunit alpha/gamma (Ab-467/199) 167 6 6 11 AKT1/2/3 (Ab-315) 168 6 6 12 MKK3/MAP2K3 (Ab-222) 169 6 6 13 PKC beta/PKCB (Ab-661) 170 6 6 14 Raf1 (Ab-289) 171 6 6 15 Src (Ab-75) 172 6 6 16 GRB2 (Ab-159) 173 6 6 17 MAPKAPK2 (Ab-272) 174 6 6 18 PLC-gamma 1 (Ab-771) 175 6 6 19 PLC-gamma 1 (Ab-783) 176 6 6 20 PLC-gamma 2 (Ab-753) 177 6 6 21 PLC-gamma 2 (Ab-1217) 178 6 6 22 VEGF R2 (Ab-1054) 179 6 6 23 ERK1/2 (N-term) 180 6 6 24 HSP90A (C-term) 181 6 6 25 RAS(p21 H and K) (inter) 182 6 6 26 IP3KA (C-term) 183 6 6 27 IP3KC (inter) 184 6 6 28 RASE (inter) 185 6 6 29 Beta actin A 6 6 30 GAPDH G 6 6 31 Negative Control N 6 6 32 Positive Marker P